Procedure for collection of nasopharyngeal swab, nasopharyngeal wash or guttural pouch wash specimens for Streptococcus equi PCR testing

written by Dr. Jeff Cook, Rood & Riddle Equine Hospital, Lexington KY (859) 233-0371 updated March 2011

 

NASOPHARYNGEAL SWAB

Supplies needed:

  • 2 people including handler

  • swab should be at least 16” long with a Dacron tip (eg. uterine swab)

  • 10 ml red top tube (sterile) containing 5 ml sterile water or transport media, without charcoal

  • exam gloves

Procedure:

  • Restrain horse (preferably with lip chain). Clean lip chain between horses first with Nolvasan solution and then with clean water.

  • Pass Dacron-tipped applicator up one nostril into the pharyngeal region.

  • Repeat for the other nostril.

  • Cut off applicator end and place tip into red top tube with water or transport media.

 

NASOPHARYNGEAL WASH

Supplies needed:

  • 3 people including handler

  • 60 cc syringe filled with sterile water Use warm water in cold weather and always for foals.

  • single use cup to catch water from nostrils

  • Kendal Sovereign 22 inch 10 french plastic catheter (single use)

  • 50 ml conical centrifuge tube (sterile) or other sterile container

  • zippered baggies

  • exam gloves for the person holding the cup & the one passing the tube; change gloves between horses

 

Procedure:

  • Restrain horse (preferably with lip chain). Clean lip chain between horses first with Nolvasan solution and then with clean water.

  • Pass catheter attached to the syringe (containing the sterile water) up one nostril up to the pharyngeal region.

  • With horses’ head relaxed, push the syringe full of water through the catheter into the pharyngeal region.

  • As the water exits both nostrils, catch as much as possible into the cup. Repeat wash if water is not flowing from both nostrils.

  • Transfer water to conical tube or container and cap tightly. Place in individual zippered bag.

 

GUTTURAL POUCH WASH

Supplies needed:

  • 3 people including handler

  • one meter endoscope with biopsy port blunt stylet***

  • 60 ml sterile water ** (see above)

  • Mila EDC 190 endoscope delivery catheter (single use)

  • 50 ml conical centrifuge tube (sterile) or other sterile container (eg. urine cup)

  • zippered baggies

  • exam gloves; change gloves between horses

 

Procedure:

  • Sedate horse (150 mg xylazine works well for adults).

  • Pass endoscope into left guttural pouch.

  • Pass Mila catheter through biopsy port into guttural pouch.

  • Irrigate pouch with 60 ml sterile water while keeping the horse’s head up to prevent the water from running out.

  • Water will pool in the medial compartment of the pouch where it can be suctioned through the Mila catheter into the syringe.

  • Transfer sample from the syringe into the conical tube or container and cap tightly. Place in individual zippered bag.

  • Repeat for right guttural pouch; combine 30 ml from each pouch wash to submit to lab.

*** Between horses, clean outside of scope, biopsy port & stylet; first with gluteraldehyde solution

(eg. Metracide 14 or Cidex), followed by dilute Nolvasan solution and finally with clean water.

 

 

Submit to lab as soon as possible. Avoid extended periods at high temperatures and refrigerate if delay occurs. Urine cups do not transport well due to their tendency to leak and crack. Each sample should be individually contained within its own zippered bag to avoid cross contamination. Please include a cold pack in shipping container.

A Printable PDF version is available here.

Last Updated on Friday, 19 August 2011 21:15
 
Procedure for collection of nasopharyngeal swab, nasopharyngeal wash or guttural pouch wash specimens for Streptococcus equi PCR testing

written by Dr. Jeff Cook, Rood & Riddle Equine Hospital, Lexington KY (859) 233-0371 updated March 2011

 

NASOPHARYNGEAL SWAB

Supplies needed:

  • 2 people including handler

  • swab should be at least 16” long with a Dacron tip (eg. uterine swab)

  • 10 ml red top tube (sterile) containing 5 ml sterile water or transport media, without charcoal

  • exam gloves

Procedure:

  • Restrain horse (preferably with lip chain). Clean lip chain between horses first with Nolvasan solution and then with clean water.

  • Pass Dacron-tipped applicator up one nostril into the pharyngeal region.

  • Repeat for the other nostril.

  • Cut off applicator end and place tip into red top tube with water or transport media.

 

NASOPHARYNGEAL WASH

Supplies needed:

  • 3 people including handler

  • 60 cc syringe filled with sterile water Use warm water in cold weather and always for foals.

  • single use cup to catch water from nostrils

  • Kendal Sovereign 22 inch 10 french plastic catheter (single use)

  • 50 ml conical centrifuge tube (sterile) or other sterile container

  • zippered baggies

  • exam gloves for the person holding the cup & the one passing the tube; change gloves between horses

 

Procedure:

  • Restrain horse (preferably with lip chain). Clean lip chain between horses first with Nolvasan solution and then with clean water.

  • Pass catheter attached to the syringe (containing the sterile water) up one nostril up to the pharyngeal region.

  • With horses’ head relaxed, push the syringe full of water through the catheter into the pharyngeal region.

  • As the water exits both nostrils, catch as much as possible into the cup. Repeat wash if water is not flowing from both nostrils.

  • Transfer water to conical tube or container and cap tightly. Place in individual zippered bag.

 

GUTTURAL POUCH WASH

Supplies needed:

  • 3 people including handler

  • one meter endoscope with biopsy port blunt stylet***

  • 60 ml sterile water ** (see above)

  • Mila EDC 190 endoscope delivery catheter (single use)

  • 50 ml conical centrifuge tube (sterile) or other sterile container (eg. urine cup)

  • zippered baggies

  • exam gloves; change gloves between horses

 

Procedure:

  • Sedate horse (150 mg xylazine works well for adults).

  • Pass endoscope into left guttural pouch.

  • Pass Mila catheter through biopsy port into guttural pouch.

  • Irrigate pouch with 60 ml sterile water while keeping the horse’s head up to prevent the water from running out.

  • Water will pool in the medial compartment of the pouch where it can be suctioned through the Mila catheter into the syringe.

  • Transfer sample from the syringe into the conical tube or container and cap tightly. Place in individual zippered bag.

  • Repeat for right guttural pouch; combine 30 ml from each pouch wash to submit to lab.

*** Between horses, clean outside of scope, biopsy port & stylet; first with gluteraldehyde solution

(eg. Metracide 14 or Cidex), followed by dilute Nolvasan solution and finally with clean water.

 

 

Submit to lab as soon as possible. Avoid extended periods at high temperatures and refrigerate if delay occurs. Urine cups do not transport well due to their tendency to leak and crack. Each sample should be individually contained within its own zippered bag to avoid cross contamination. Please include a cold pack in shipping container.

A Printable PDF version is available here.

Last Updated on Friday, 19 August 2011 21:15