Services

EDS Services List (Rev. 01/11/2017)

EDS Test Request Form (Rev. 01/01/2018)

EDS Test Descriptions and Specimen Guidelines


Equine Protozoal Myeloencephalitis (EPM):

Sarcocystis neurona SAG 2/3/4 ELISA

This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky’s Gluck Equine Research Center and is available exclusively at EDS.  An endpoint titer provides quantified results based on the IgG response to three S. neurona specific antigens.  These antigens have been shown to correlate well with the standard western blot, clinical diagnosis and necropsy.

Serum can be tested alone; however the interpretation of serum results is to confirm exposure to S. neurona.  A negative serum result is a good rule out for EPM caused by S. neurona.  EDS archives sample for up to 2 years, so comparison testing of serum samples from different collection dates is available.  Submit 1ml of serum from a red top tube or serum separator tube.

Paired serum and cerebrospinal fluid (CSF) are the recommended samples. Serum:CSF titer ratios are highly predictive for distinguishing EPM from other neurologic diseases.  The titer ratio is based on the normal passive movement of antibody across the blood brain barrier at a serum:CSF ratio of 100.  If antibodies are being produced intrathecally, this ratio will be less than 100 indicating an active immune response to the S. neurona parasite.  Submit 1ml serum from a red top tube and 1ml of CSF in a red top tube.

Sarcocystis neurona western blot

The S. neurona standard western blot performed at EDS is based on the method developed and validated at the Gluck Equine Research Center at the University of Kentucky (Dr. David Granstrom 1993).  It detects the IgG antibody response to specific S. neurona antigens resulting from exposure to and infection by the parasite.  Results are semi-quantified (positive, low positive, weak positive, negative) based on intensity and the specific antigen banding patterns as compared to control sera.  EDS lab recommends the SAG 2/4/3 ELISA for testing CSF.  Submit 1ml of serum from a red top tube.

Sarcocystis neurona PCR

The PCR test detects S.neurona DNA and thus the actual presence of the parasite.  This assay is performed on CSF and is best used in conjunction with the western blot  or ELISA test.  Submit 1ml CSF in EDTA purple top tube.

Neospora hughesi ELISA

The Neospora hughesi ELISA was developed in the lab of Dr. Dan Howe at the University of Kentucky's Gluck Equine Research center.  Serum and/or CSF samples can be tested for an IgG response to the N. hughesi parasite.   The ELISA generates an endpoint titer and paired serum and CSF samples can be used to calculate a Serum:CSF titer ratio which is predictive for an EPM diagnosis.  Submit 1ml serum from a red top tube and/or 1ml CSF in red top tube.

S. neurona specific index

The specific index is performed on paired serum and CSF samples in conjunction with the S. neurona SAG 2/3/4 ELISA.  This test can help discern an intrathecal IgG response from contamination by utilizing a ratio of serum and CSF albumin and IgG levels.  It is very helpful in the interpretation of results when the CSF has been contaminated with blood or when the Serum:CSF ratio is equal to 100.  Submit 1ml serum from a red top tube and 1ml of CSF in a red top tube.

Streptococcus equi (strangles):

Streptococcus equi real-time PCR

The PCR test detects S. equi bacterial DNA and is used primarily to identify asymptomatic carriers.  EDS lab's PCR method employs two separate gene targets in the PCR reaction for improved specificity and sensitivity of test results.  This PCR is an effective screening tool for systematically evaluating and managing resident and new horses on a property to control and prevent potential outbreaks.  Nasal washes, nasal swabs or guttural pouch washes are appropriate samples for the S. equi PCR.   Back up culture is also available.  Submit 10-30ml nasal wash or guttural pouch wash in screw cap conical tube; or nasal swabs in a red top tube (dry or in 0.5ml sterile saline).

Streptococcus equi SEM ELISA

The SeM ELISA test was developed at the Gluck Equine Research Center at the University of Kentucky (Dr. John Timoney) and assesses the IgG endpoint titer against the highly immunogenic S. equi specific M protein.  It is helpful for making vaccination decisions on horses with existing titers, identifying horses at risk of complications due to elevated titers, or for horses with aberrant abscesses (bastard strangles).  The SEM ELISA should not be used to determine the infection status of horses with clinical symptoms of strangles.  Submit 1ml serum from red top tube.

Equine Herpesvirus (EHV):

EHV-1 real-time PCR

This PCR test detects EHV-1 strains associated with respiratory disease, abortion, and will also distinguish if the ORF30 (DNA polymerase gene) mutation is present.  The presence of this mutation has a high correlation with the neurological form of this disease, equine herpes myeloencephalopathy (EHM).   Submit 5-10ml nasal wash in conical tube, or nasal swab in a red top tube (dry or in 0.5ml sterile saline); or 7ml whole blood in EDTA purple top tube. Submission of both nasal wash/nasal swab and EDTA whole blood is recommended.

EHV-4 real-time PCR

This PCR test detects EHV-4 strains which are generally associated with respiratory disease. Submit a nasal swab in a red top tube  (dry or in 0.5ml sterile saline)

Rhodococcus equi real-time PCR:

The PCR test identifies strains of R. equi by detecting the virulence plasmid gene vapA.  The vapA gene is highly associated with the ability to cause clinical disease and pneumonia in foals.  A trans-tracheal wash is the recommended specimen for R. equi PCR, however, a nasal swab or fecal sample can also be tested.  Back up culture is also available.  Submit 2-5ml trans-tracheal wash in conical tube or red top tube; or nasal swab in a red top tube (dry or in 0.5ml sterile saline); or at least 10g feces in screw top cup.

Salmonella spp. real-time PCR:

The PCR test detects DNA from pathogenic species of Salmonella and can be used as a bio-surveillance tool for the identification of asymptomatic/shedding horses.   This assay is also useful for the monitoring of in-patients in a hospital setting.  Feces or a fecal swab is the recommended specimen; EDS lab is also able to test environmental swabs for Salmonella species.  Submit at least 10g feces or fecal swab in red top tube. For environmental samples submit 1 swiffer/dry wipe per area to be tested in a zipper bag.

Equine Influenza Virus (EIV) real-time PCR:

This test detects the H3N8 and H7N7 (New York) strains of EIV and a nasal swab is the recommended sample.  EIV PCR is included in the respiratory panels.   Submit nasal swab in a red top tube (dry or in 0.5ml sterile saline).

Equine Rhinitis Virus A and B real-time PCR:

Equine Rhinitis Virus type A and type B are both detected by this test.  Urine is the recommended sample for this respiratory pathogen as shedding by the urinary tract has a longer detection window than in nasal secretions; a nasal swab or wash can also be tested.  This test is part of our expanded respiratory panel.  Submit 2-5ml urine in red top tube or screw cap conical tube; or nasal swab in red top tube (dry or in 0.5ml sterile saline)

Equine Arteritis Virus (EAV) real-time PCR:

EAV RNA is detected by this PCR test.  Arteritis virus is associated with respiratory disease in horses but  more significantly may be associated with abortions.  Appropriate specimens for submission include nasal swabs or washes, semen,  whole blood (EDTA), or ocular swabs.  PCR is not an accepted test method for export.  This test is part of our expanded respiratory panel.  Submit nasal swab in red top tube (dry or in 0.5ml sterile saline); or 10-30ml nasal wash in screw cap conical tube; or 2-5ml semen in red top tube; or 7ml whole blood in EDTA purple top tube; or ocular swab in red top tube (dry or in 0.5ml sterile saline).

Lawsonia intracellularis ELISA:

This assay was developed by Dr. Allen Page in the lab of Dr. Dave Horohov at the University of Kentucky's Gluck Equine Research Center.  It is available exclusively at EDS.  To support diagnosis of equine proliferative enteropathy (EPE) the presence of serum antibodies, with concurrent hypoalbuminemia or hypoproteinemia, indicates a recent or current infection.  Submit 1ml serum from red top tube.

West Nile Virus (WNV) IgM ELISA:

The IgM ELISA test is used to detect recent exposure to the west nile virus.  Presence of IgM antibodies against WNV are diagnostic of current or recent infection.  This assay is included in our neurologic panels.  Submit 1ml serum from red top tube.

Equine Infectious Anemia Virus (EIAV):

EIA AGID and EIA ELISA

EDS is a federally accredited lab for this regulated testing and offers both approved test formats:   AGID (Coggins) and ELISA.  Our laboratory is now accepting electronic EIA forms submitted through the USDA/APHIS Veterinary Process streaming (VSPS) portal.  The electronic service is free to accredited veterinarians and laboratories.  Please submit a fully completed and signed form with each sample.  Submit 7ml blood in red top tube along with completed manual form or print out of electronic form.

Panels and Profiles:

Several combinations of different, but complementary, tests are also available.  Please refer to the service list for a complete listing.  The most commonly ordered profiles are listed below along with the specimen requirements.

S. neurona SAG 2/4/3 ELISA titer ratio (EPM): 1ml serum from red top tube and 1ml CSF in red top tube

Neurologic Panel II (EPM, WNV and EHV 1): 1ml serum from red top tube, and 7ml whole blood in EDTA purple top tube or nasal swab in red top tube (dry or in 0.5ml saline)

Respiratory Panel by real time PCR (S. equi, EHV1, EHV4, EIV): Nasal swab in red top tube (dry or in 0.5ml sterile saline, or 10-30ml nasal wash in screw cap conical tube.

 

Several combinations of different, but complementary, tests are also available. Please refer to the service list for a complete listing.

 

Test Request Form (Rev. 01/01/2018)

 

Last Updated on Friday, 09 March 2018 13:06
 
Services

EDS Services List (Rev. 01/11/2017)

EDS Test Request Form (Rev. 01/01/2018)

EDS Test Descriptions and Specimen Guidelines


Equine Protozoal Myeloencephalitis (EPM):

Sarcocystis neurona SAG 2/3/4 ELISA

This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky’s Gluck Equine Research Center and is available exclusively at EDS.  An endpoint titer provides quantified results based on the IgG response to three S. neurona specific antigens.  These antigens have been shown to correlate well with the standard western blot, clinical diagnosis and necropsy.

Serum can be tested alone; however the interpretation of serum results is to confirm exposure to S. neurona.  A negative serum result is a good rule out for EPM caused by S. neurona.  EDS archives sample for up to 2 years, so comparison testing of serum samples from different collection dates is available.  Submit 1ml of serum from a red top tube or serum separator tube.

Paired serum and cerebrospinal fluid (CSF) are the recommended samples. Serum:CSF titer ratios are highly predictive for distinguishing EPM from other neurologic diseases.  The titer ratio is based on the normal passive movement of antibody across the blood brain barrier at a serum:CSF ratio of 100.  If antibodies are being produced intrathecally, this ratio will be less than 100 indicating an active immune response to the S. neurona parasite.  Submit 1ml serum from a red top tube and 1ml of CSF in a red top tube.

Sarcocystis neurona western blot

The S. neurona standard western blot performed at EDS is based on the method developed and validated at the Gluck Equine Research Center at the University of Kentucky (Dr. David Granstrom 1993).  It detects the IgG antibody response to specific S. neurona antigens resulting from exposure to and infection by the parasite.  Results are semi-quantified (positive, low positive, weak positive, negative) based on intensity and the specific antigen banding patterns as compared to control sera.  EDS lab recommends the SAG 2/4/3 ELISA for testing CSF.  Submit 1ml of serum from a red top tube.

Sarcocystis neurona PCR

The PCR test detects S.neurona DNA and thus the actual presence of the parasite.  This assay is performed on CSF and is best used in conjunction with the western blot  or ELISA test.  Submit 1ml CSF in EDTA purple top tube.

Neospora hughesi ELISA

The Neospora hughesi ELISA was developed in the lab of Dr. Dan Howe at the University of Kentucky's Gluck Equine Research center.  Serum and/or CSF samples can be tested for an IgG response to the N. hughesi parasite.   The ELISA generates an endpoint titer and paired serum and CSF samples can be used to calculate a Serum:CSF titer ratio which is predictive for an EPM diagnosis.  Submit 1ml serum from a red top tube and/or 1ml CSF in red top tube.

S. neurona specific index

The specific index is performed on paired serum and CSF samples in conjunction with the S. neurona SAG 2/3/4 ELISA.  This test can help discern an intrathecal IgG response from contamination by utilizing a ratio of serum and CSF albumin and IgG levels.  It is very helpful in the interpretation of results when the CSF has been contaminated with blood or when the Serum:CSF ratio is equal to 100.  Submit 1ml serum from a red top tube and 1ml of CSF in a red top tube.

Streptococcus equi (strangles):

Streptococcus equi real-time PCR

The PCR test detects S. equi bacterial DNA and is used primarily to identify asymptomatic carriers.  EDS lab's PCR method employs two separate gene targets in the PCR reaction for improved specificity and sensitivity of test results.  This PCR is an effective screening tool for systematically evaluating and managing resident and new horses on a property to control and prevent potential outbreaks.  Nasal washes, nasal swabs or guttural pouch washes are appropriate samples for the S. equi PCR.   Back up culture is also available.  Submit 10-30ml nasal wash or guttural pouch wash in screw cap conical tube; or nasal swabs in a red top tube (dry or in 0.5ml sterile saline).

Streptococcus equi SEM ELISA

The SeM ELISA test was developed at the Gluck Equine Research Center at the University of Kentucky (Dr. John Timoney) and assesses the IgG endpoint titer against the highly immunogenic S. equi specific M protein.  It is helpful for making vaccination decisions on horses with existing titers, identifying horses at risk of complications due to elevated titers, or for horses with aberrant abscesses (bastard strangles).  The SEM ELISA should not be used to determine the infection status of horses with clinical symptoms of strangles.  Submit 1ml serum from red top tube.

Equine Herpesvirus (EHV):

EHV-1 real-time PCR

This PCR test detects EHV-1 strains associated with respiratory disease, abortion, and will also distinguish if the ORF30 (DNA polymerase gene) mutation is present.  The presence of this mutation has a high correlation with the neurological form of this disease, equine herpes myeloencephalopathy (EHM).   Submit 5-10ml nasal wash in conical tube, or nasal swab in a red top tube (dry or in 0.5ml sterile saline); or 7ml whole blood in EDTA purple top tube. Submission of both nasal wash/nasal swab and EDTA whole blood is recommended.

EHV-4 real-time PCR

This PCR test detects EHV-4 strains which are generally associated with respiratory disease. Submit a nasal swab in a red top tube  (dry or in 0.5ml sterile saline)

Rhodococcus equi real-time PCR:

The PCR test identifies strains of R. equi by detecting the virulence plasmid gene vapA.  The vapA gene is highly associated with the ability to cause clinical disease and pneumonia in foals.  A trans-tracheal wash is the recommended specimen for R. equi PCR, however, a nasal swab or fecal sample can also be tested.  Back up culture is also available.  Submit 2-5ml trans-tracheal wash in conical tube or red top tube; or nasal swab in a red top tube (dry or in 0.5ml sterile saline); or at least 10g feces in screw top cup.

Salmonella spp. real-time PCR:

The PCR test detects DNA from pathogenic species of Salmonella and can be used as a bio-surveillance tool for the identification of asymptomatic/shedding horses.   This assay is also useful for the monitoring of in-patients in a hospital setting.  Feces or a fecal swab is the recommended specimen; EDS lab is also able to test environmental swabs for Salmonella species.  Submit at least 10g feces or fecal swab in red top tube. For environmental samples submit 1 swiffer/dry wipe per area to be tested in a zipper bag.

Equine Influenza Virus (EIV) real-time PCR:

This test detects the H3N8 and H7N7 (New York) strains of EIV and a nasal swab is the recommended sample.  EIV PCR is included in the respiratory panels.   Submit nasal swab in a red top tube (dry or in 0.5ml sterile saline).

Equine Rhinitis Virus A and B real-time PCR:

Equine Rhinitis Virus type A and type B are both detected by this test.  Urine is the recommended sample for this respiratory pathogen as shedding by the urinary tract has a longer detection window than in nasal secretions; a nasal swab or wash can also be tested.  This test is part of our expanded respiratory panel.  Submit 2-5ml urine in red top tube or screw cap conical tube; or nasal swab in red top tube (dry or in 0.5ml sterile saline)

Equine Arteritis Virus (EAV) real-time PCR:

EAV RNA is detected by this PCR test.  Arteritis virus is associated with respiratory disease in horses but  more significantly may be associated with abortions.  Appropriate specimens for submission include nasal swabs or washes, semen,  whole blood (EDTA), or ocular swabs.  PCR is not an accepted test method for export.  This test is part of our expanded respiratory panel.  Submit nasal swab in red top tube (dry or in 0.5ml sterile saline); or 10-30ml nasal wash in screw cap conical tube; or 2-5ml semen in red top tube; or 7ml whole blood in EDTA purple top tube; or ocular swab in red top tube (dry or in 0.5ml sterile saline).

Lawsonia intracellularis ELISA:

This assay was developed by Dr. Allen Page in the lab of Dr. Dave Horohov at the University of Kentucky's Gluck Equine Research Center.  It is available exclusively at EDS.  To support diagnosis of equine proliferative enteropathy (EPE) the presence of serum antibodies, with concurrent hypoalbuminemia or hypoproteinemia, indicates a recent or current infection.  Submit 1ml serum from red top tube.

West Nile Virus (WNV) IgM ELISA:

The IgM ELISA test is used to detect recent exposure to the west nile virus.  Presence of IgM antibodies against WNV are diagnostic of current or recent infection.  This assay is included in our neurologic panels.  Submit 1ml serum from red top tube.

Equine Infectious Anemia Virus (EIAV):

EIA AGID and EIA ELISA

EDS is a federally accredited lab for this regulated testing and offers both approved test formats:   AGID (Coggins) and ELISA.  Our laboratory is now accepting electronic EIA forms submitted through the USDA/APHIS Veterinary Process streaming (VSPS) portal.  The electronic service is free to accredited veterinarians and laboratories.  Please submit a fully completed and signed form with each sample.  Submit 7ml blood in red top tube along with completed manual form or print out of electronic form.

Panels and Profiles:

Several combinations of different, but complementary, tests are also available.  Please refer to the service list for a complete listing.  The most commonly ordered profiles are listed below along with the specimen requirements.

S. neurona SAG 2/4/3 ELISA titer ratio (EPM): 1ml serum from red top tube and 1ml CSF in red top tube

Neurologic Panel II (EPM, WNV and EHV 1): 1ml serum from red top tube, and 7ml whole blood in EDTA purple top tube or nasal swab in red top tube (dry or in 0.5ml saline)

Respiratory Panel by real time PCR (S. equi, EHV1, EHV4, EIV): Nasal swab in red top tube (dry or in 0.5ml sterile saline, or 10-30ml nasal wash in screw cap conical tube.

 

Several combinations of different, but complementary, tests are also available. Please refer to the service list for a complete listing.

 

Test Request Form (Rev. 01/01/2018)

 

Last Updated on Friday, 09 March 2018 13:06